Barrie Ingham

Basil of Baker Street / Bartholomew (voice)

Vincent Price

Professor Ratigan (voice)

Val Bettin

Dr. David Q. Dawson / Thug Guard (voice)

Candy Candido

Fidget (voice)

Eve Brenner

The Mouse Queen (voice)

Alan Young

Mr. Flaversham (voice)

Basil Rathbone

Sherlock Holmes (voice)

Walker Edmiston

Citizen / Thug Guard (voice)

Wayne Allwine

Thug Guard (voice)

Tony Anselmo

Thug Guard (voice)

Frank Welker

Toby the Dog / Felicia the Cat (voice) (uncredited)

Laurie Main

Dr. Watson (archive sound)

Shani Wallis

Lady Mouse (voice)

Melissa Manchester

Miss Kitty Mouse (voice) (uncredited)

Charles Fleischer

Additional Voices (voice) (uncredited)